pco.edge Series
개요
sCMOS(Scientific CMOS) 카메라
전통적인 CCD 이미지 센서와, CMOS 센서의 장점을 합쳐 놓은 새로운 센서의 종류
장점 - 고해상도(4.2Mpxiel @100fps), 높은 QE(~95%), 낮은 판독노이즈(0.8e-), 16bit High Dynamic range
UV~NIR 영역대 까지 커버 가능( 180nm ~ 1050nm)
단점 - CMOS센서 대비 고가(ICCD에 비해서는 저렴)
적용가능 어플리케이션
Highspeed Microscopy Localization Microscopy
Lightsheet Microscopy Confocal Microscopy
Digital Pathology Syncrotron Radiation
3D Measurements Flow Visualization
Structured Illumination Microscopy Light Microscopy 외
고해상도 & 고감도를 필요로 하는 형광실험 @일반 형광용 모노카메라에 비해 몇배 짧은 노출가능
빠른 형광 이미징(미세유체셀 MEMS, 바이오칩) @ 4.2Mpxiel 에서 100fps 속도
NIR 영역대의 고감도 이미징(Si 투과, VCSEL 투과 이미징)
UV 영역대의 고감도 이미징(이물 판독, 코로나방전 촬영)
Coolded sCMOS Camera
pco.edge 26
- true charge domain global shutter
- 5120 x 5120 pixel resolution
- down to -20°C adjustable cooling
- compact design
- up to 72 % quantum efficiency
- USB 3.1 Gen1
pco.edge 5.5
- 1.0 e- med readout noise
- 2560 x 2160 pixel resolution
- 30 000 : 1 dynamic range
- 100 fps maximum frame rate
- rolling & global shutter functionality
- Camera Link HS or USB 3.0
pco.edge 4.2 bi UV
- 190 nm - 1100 nm spectral range
- back illuminated sensor
- up to 95 % quantum efficiency
- down to -25°C adjustable cooling
- 2048 x 2048 pixel resolution
- USB 3.1 Gen1
pco.edge 4.2 bi
- back illuminated sensor
- up to 95 % quantum efficiency
- down to -25°C adjustable cooling
- 2048 x 2048 pixel resolution
- 40 fps maximum frame rate
- USB 3.1 Gen1
pco.edge 4.2
- 0.8 e- med readout noise
- 2048 x 2048 pixel resolution
- up to 37 500 : 1 dynamic range
- 100 fps maximum frame rate
- up to 82 % quantum efficiency
- Camera Link HS or USB 3.0
pco.edge 4.2 LT
- 0.8 e- med readout noise
- 2048 x 2048 pixel resolution
- 37 500 : 1 dynamic range
- 40 fps maximum frame rate
- up to 82 % quantum efficiency
- USB 3.0 interface
pco.edge 3.1
- 1.1 e- med readout noise
- 2048 x 1536 pixel resolution
- 27 000 : 1 dynamic range
- rolling & global shutter functionality
- USB 3.0 interface
Compact sCMOS Camera
pco.panda 26
- available in mono and color
- true charge domain global shutter
- 5120 x 5120 pixel resolution
- dust-protected housing
- ultra-compact design
- up to 65 % quantum efficiency
pco.panda 4.2 bi UV
- 190 nm - 1100 nm spectral range
- back illuminated sensor
- ultra-compact design
- 2048 x 2048 pixel
- USB 3.1 Gen1
pco.panda 4.2 bi
- back illuminated sensor
- up to 95 % quantum efficiency
- ultra-compact design
- 2048 x 2048 pixel
- USB 3.1 Gen1
pco.panda 4.2
- 2.1 e- med readout noise
- up to 80 % quantum efficiency
- ultra-compact design
- 2048 x 2048 pixel
- USB 3.1 Gen1
스펙
Rolling shutter readout modes - optimized for synchronization of microscopes and scanning applications
All pco.edge sCMOS cameras from the beginning feature a variety of precise synchronization modes, which are optimized for advanced microscopy im- aging and scanning. The flexible frame and line trig- gers with very low latency in combination with the free selectable readout modes can easily be com- bined to cover every modern microscopy situation to name a few:
- lightsheet microscopy
- selective plane imaging microscopy (SPIM)
- structured illumination microscopy
- localizations microscopy (GSD, PALM, STORM, dSTORM)
- spinning disk confocal microscopy
- RESOLFT
For example, one mode is used in a lightsheet or SPIM application, the lower right rolling shutter operational mode “single top down” operation is convenient to properly synchronize the camera exposure with the scanner. On the other hand, if speed is required and a flash like exposure is applied the upper left mode “dual outside in” is used for localization microscopy techniques like GSD, PALM or STORM.
Superior image quality
The pco.edge sCMOS camera features outstand- ing low read out noise. Even at maximum speed of 40 frames/s at full resolution of 2048 x 2048 pixel the noise is 0.8 e- med. Moreover the pco.edge provides
an excellent homogeneous pixel response to light (PRNU, photo response non-uniformity) and an excel- lent homogeneous dark signal pixel behaviour (DSNU, dark signal non-uniformity), which is achieved by a sophisticated electronic circuit technology and firm- ware algorithms.
The lower figure shows a comparison of a scientific grade CCD and the new pco.sCMOS image sensor under similar weak illumination conditions. This dem- onstrates the superiority of sCMOS over CCD with regards to read out noise and dynamic, without any smear (the vertical lines in the CCD image).
Dark image comparison with the measured distribution of “hot blinking” pixels at typical cooled down operating temperature of the image sensor. The left image gives a 3D view with the sophisticated “blinker filter” algorithm off and the right image shows the result with the filter switched on.
The left image was recorded by a scientific CCD camera while the right image was recorded by a pco.edge under identical conditions.
Flexibility and free of latency
User selectable choice of rolling shutter modes for exposure provides flexibility for a wide range of applications. The advantages of rolling shutter are high frame rates and low read out noise. Due to realtime transmission of the image data to the PC, there is no latency between recording and access or storage of the data.
37 500:1 dynamic range
Due to the excellent low noise and the high fullwell capacity of the sCMOS image sensor an intra scene dynamic range of better than 37 500 : 1 is achieved. A unique architecture of dual column level amplifiers and dual 11 bit ADCs is designed to maximize dynamic range and to minimize read out noise simultaneously. Both ADC values are analyzed and merged into one high dynamic 16 bit value.
the top image shows an extract of a typical pco.edge recording of a grey scale with a 1 : 10 000 dynamic in 20 steps. The bottom image is a plot of the grey values profile along the centered line through the top image (with gamma 2.2).
pco.edge Series
개요
sCMOS(Scientific CMOS) 카메라
전통적인 CCD 이미지 센서와, CMOS 센서의 장점을 합쳐 놓은 새로운 센서의 종류
장점 - 고해상도(4.2Mpxiel @100fps), 높은 QE(~95%), 낮은 판독노이즈(0.8e-), 16bit High Dynamic range
UV~NIR 영역대 까지 커버 가능( 180nm ~ 1050nm)
단점 - CMOS센서 대비 고가(ICCD에 비해서는 저렴)
적용가능 어플리케이션
Highspeed Microscopy Localization Microscopy
Lightsheet Microscopy Confocal Microscopy
Digital Pathology Syncrotron Radiation
3D Measurements Flow Visualization
Structured Illumination Microscopy Light Microscopy 외
고해상도 & 고감도를 필요로 하는 형광실험 @일반 형광용 모노카메라에 비해 몇배 짧은 노출가능
빠른 형광 이미징(미세유체셀 MEMS, 바이오칩) @ 4.2Mpxiel 에서 100fps 속도
NIR 영역대의 고감도 이미징(Si 투과, VCSEL 투과 이미징)
UV 영역대의 고감도 이미징(이물 판독, 코로나방전 촬영)
Coolded sCMOS Camera
pco.edge 26
pco.edge 5.5
pco.edge 4.2 bi UV
pco.edge 4.2 bi
pco.edge 4.2
pco.edge 4.2 LT
pco.edge 3.1
Compact sCMOS Camera
pco.panda 26
pco.panda 4.2 bi UV
pco.panda 4.2 bi
pco.panda 4.2
스펙
Rolling shutter readout modes - optimized for synchronization of microscopes and scanning applications
All pco.edge sCMOS cameras from the beginning feature a variety of precise synchronization modes, which are optimized for advanced microscopy im- aging and scanning. The flexible frame and line trig- gers with very low latency in combination with the free selectable readout modes can easily be com- bined to cover every modern microscopy situation to name a few:
- lightsheet microscopy
- selective plane imaging microscopy (SPIM)
- structured illumination microscopy
- localizations microscopy (GSD, PALM, STORM, dSTORM)
- spinning disk confocal microscopy
- RESOLFT
For example, one mode is used in a lightsheet or SPIM application, the lower right rolling shutter operational mode “single top down” operation is convenient to properly synchronize the camera exposure with the scanner. On the other hand, if speed is required and a flash like exposure is applied the upper left mode “dual outside in” is used for localization microscopy techniques like GSD, PALM or STORM.
Superior image quality
The pco.edge sCMOS camera features outstand- ing low read out noise. Even at maximum speed of 40 frames/s at full resolution of 2048 x 2048 pixel the noise is 0.8 e- med. Moreover the pco.edge provides
an excellent homogeneous pixel response to light (PRNU, photo response non-uniformity) and an excel- lent homogeneous dark signal pixel behaviour (DSNU, dark signal non-uniformity), which is achieved by a sophisticated electronic circuit technology and firm- ware algorithms.
The lower figure shows a comparison of a scientific grade CCD and the new pco.sCMOS image sensor under similar weak illumination conditions. This dem- onstrates the superiority of sCMOS over CCD with regards to read out noise and dynamic, without any smear (the vertical lines in the CCD image).
Dark image comparison with the measured distribution of “hot blinking” pixels at typical cooled down operating temperature of the image sensor. The left image gives a 3D view with the sophisticated “blinker filter” algorithm off and the right image shows the result with the filter switched on.
The left image was recorded by a scientific CCD camera while the right image was recorded by a pco.edge under identical conditions.
Flexibility and free of latency
User selectable choice of rolling shutter modes for exposure provides flexibility for a wide range of applications. The advantages of rolling shutter are high frame rates and low read out noise. Due to realtime transmission of the image data to the PC, there is no latency between recording and access or storage of the data.
37 500:1 dynamic range
Due to the excellent low noise and the high fullwell capacity of the sCMOS image sensor an intra scene dynamic range of better than 37 500 : 1 is achieved. A unique architecture of dual column level amplifiers and dual 11 bit ADCs is designed to maximize dynamic range and to minimize read out noise simultaneously. Both ADC values are analyzed and merged into one high dynamic 16 bit value.
the top image shows an extract of a typical pco.edge recording of a grey scale with a 1 : 10 000 dynamic in 20 steps. The bottom image is a plot of the grey values profile along the centered line through the top image (with gamma 2.2).